Interactions between enzymes and oxide colloidal particles and their influence on enzymatic activity

Ludmilla Derr

doi:10.51202/9783185760051

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Bibliographic data

Copyright year: 2016
ISBN-Print: 978-3-18-376005-3
ISBN-Online: 978-3-18-576005-1
Publisher: VDI Verlag, Düsseldorf
Series: Grund- und Werkstoffe/Kunststoffe
Volume: 760
Language: German
Pages: 194
Product type: Book Titles

Titelei/Inhaltsverzeichnis Partial access Pages I - XX Download chapter (PDF)
1. Aims and motivation No access
1. Van der Waals forces No access
2. 1. Surface charge No access
  2. Zeta potential No access
  3. The Poisson-Boltzmann equation No access
  4. The electrostatic interaction energy between two spheres No access
3. The DLVO theory No access
4. Non-DLVO forces No access
1. The amino acid composition of proteins No access
2. Protein structure No access
3. 1. a-Chymotrypsin No access
1. Alumina colloidal particles No access
2. Silica colloidal particles No access
3. Titania colloidal particles No access
4. Surface functionalization of colloidal particles No access
5. Covalent enzyme immobilization No access
1. Influence on protein-particle interactions No access
2. Thermodynamic approach No access
3. 1. The Langmuir adsorption model No access
  2. Other adsorption models No access
1. Michaelis-Menten kinetics No access
2. Determination of KM and Vmax from linearization plots No access
3. Activity assays: principles No access
1. Electroacoustic spectroscopy: zeta potential No access
2. Gas adsorption isotherms: specific surface area SSABET, hydrophobicity/ hydrophilicity No access
3. UV-Vis spectroscopy: protein quantification No access
4. Activity assessment of free and adsorbed a-chymotrypsin No access
5. Matrix-assisted laser desorption ionization time-of-flight mass spectroscopy No access
6. Circular dichroism spectroscopy: changes in protein structure No access
7. Visual molecular dynamics: modeling of proteins No access
1. Introduction No access
2. 1. Materials No access
  2. Characterization of TiO2 colloidal particles No access
  3. Hydrophobic/ hydrophilic properties of chymotrypsin No access
  4. Time-dependent and pH-dependent adsorption/ desorption of chymotrypsin No access
  5. Concentration-dependent adsorption of chymotrypsin on TiO2 No access
  6. Determination of the enzymatic activity of chymotrypsin No access
  7. Statistical analysis No access
3. 1. Characterization of TiO2 colloidal particles No access
  2. Time-dependent and pH-dependent adsorption/ desorption of chymotrypsin No access
  3. Concentration-dependent adsorption of chymotrypsin on TiO2 No access
  4. Substrate concentration-dependency for free and adsorped chymotrypsin No access
4. Discussion No access
5. Conclusions No access
6. Acknowledgements No access
7. Source No access
1. Introduction No access
2. 1. Materials No access
  2. Characterization of SiO2 and Al2O3 No access
  3. Size determination and hydrophobic/ hydrophilic properties of chymotrypsin No access
  4. Adsorption and desorption studies of chymotrypsin No access
  5. Quantitative determination of adsorbed chymotrypsin No access
  6. Enzymatic activity measurements of free chymotrypsin No access
  7. Enzymatic activity measurements of adsorbed chymotrypsin No access
  8. Statistical analysis No access
3. 1. Characterization of SiO2 and Al2O3 No access
  2. Size determination and hydrophobic/ hydrophilic properties of chymotrypsin No access
  3. Adsorption of chymotrypsin on SiO2 and Al2O3 colloidal particles No access
  4. Adsorption/ desorption studies of chymotrypsin No access
  5. Enzymatic activity of adsorbed chymotrypsin No access
4. Discussion No access
5. Conclusions No access
6. Acknowledgements No access
1. Introduction No access
2. 1. Materials No access
  2. Characterization of SiO2 and TiO2 No access
  3. MALDI-ToF-MS No access
  4. Time- and pH-dependent adsorption of chymotrypsin to Sio2 and TiO2 No access
  5. Concentration-dependent adsorption of chymotrypsin to Sio2 and TiO2 No access
  6. Desorption studies No access
  7. Molecular Dynamics simulations No access
3. 1. Characterization of SiO2 and TiO2 colloidal particles No access
  2. Dependence of the protein adsorption on incubation time and pH No access
  3. MALDI-ToF-MS analysis of possible autolysis No access
  4. Analysis of the adsorption layer No access
  5. Dependence of the adsorbed amount on the protein concentration No access
  6. Longe-range interaction force of chymotrypsin over SiO2 and TiO2 No access
  7. Interaction forces for chymotrypsin multi-layers No access
  8. Explicit-solvent MD simulations of chymotrypsin adsorption on TiO2 and SiO2 No access
4. Discussion and conclusions No access
5. Acknowledgements No access
6. Appendix: Circular dichroism studies No access
7. Source No access
1. Introduction No access
2. 1. Materials No access
  2. Characterization of alumina and silica colloidal particles No access
  3. Chymotrypsin immobilization on alumina and silica colloidal particles No access
  4. Proteolysis test by MALDI-ToF-MS measurements No access
  5. Data analysis No access
3. Results No access
4. Discussion No access
5. Conclusions No access
6. Acknowledgements No access
7. Appendix: Characterization of colloidal particles after surface functionalization and enzyme immobilization using Fourier transform infrared (FTIR) spectroscopy No access
8. Source No access
Conclusions No access Pages 164 - 167
Outlook No access Pages 168 - 169
List of students projects No access Pages 170 - 170
List of publications No access Pages 171 - 171
Selected oral and poster presentations No access Pages 172 - 172
References No access Pages 173 - 194