Vergleich von Polyethersulfon- und Quarzfaserfiltern für DNA-Analysen aus Bioaerosolen/Comparison of Polyethersulfone and Quartz Fiber Filters for DNA Analyses of Bioaerosols

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Cover of Volume: Gefahrstoffe Volume 85 (2025), Edition 09-10
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Volume 85 (2025), Edition 09-10

Authors:
, , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , ,
Publisher
VDI fachmedien, Düsseldorf
Publication year
2025
ISSN-Online
0949-8036
ISSN-Print
0949-8036

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Volume 85 (2025), Edition 09-10

Vergleich von Polyethersulfon- und Quarzfaserfiltern für DNA-Analysen aus Bioaerosolen/Comparison of Polyethersulfone and Quartz Fiber Filters for DNA Analyses of Bioaerosols

Authors:
,
ISSN-Print
0949-8036
ISSN-Online
0949-8036
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For reliable risk assessment of airborne biological agents, it is essential to optimize the entire bioaerosol analytics process from collection to analysis. In this study, quartz fiber and polyethylene sulfone (PES) filters were compared with respect to their usability for DNA analyses of bioaerosol samples. No differences could be found for both materials with regard to extracted DNA amount. However, PES filters allowed the extraction of higher molecular weight DNA compared to quartz fiber filters. This is of importance within the scope of occupational safety and health, to integrate DNA sequencing technology into future risk assessments. Moreover, our results show that shorter sampling times may lead to relative higher extracted DNA concentrations.

Bibliography

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